メチル化DNA解析

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodelling, and perturbations in methylation patterns are implicated in the development of cancer, neurodegenerative diseases, and neurological disorders. Therefore, the mapping of methylated bases (the methylome) is critical to understanding gene expression and other processes subject to epigenetic regulation. Novogene provides whole genome sequencing of bisulfite-converted DNA, as an effective method to identify individually methylated cytosines on a genome-wide scale. Methylome analysis is an increasingly valuable research tool with a range of applications, including studies on gene regulation, stem cell differentiation, embryogenesis, aging, cancer and other diseases, and phenotypic diversity and evolution in plants and animals.

サービスの強み

Extensive experience: over 400 samples successfully sequenced and a bioinformatics analysis team composed entirely of Ph.D. scientists.

Unsurpassed data quality: We guarantee that ? 80% of bases have a sequencing quality score ? Q30, exceeding Illumina’s official guarantee of ? 75%.

Comprehensive data analysis: We use widely-accepted mainstream software such as Bismark and a mature in-house pipeline for mapping, DMR analysis, functional analysis and data visualization.

サービスの流れ

SEQUENCING STRATEGY: 250 bp ~ 300 bp insert DNA library; HiSeq platform, paired-end 150 bp

DATA QUALITY GUARANTEE: Q30 ? 80%

SAMPLE REQUIREMENTS: DNA amount: ? 5 μg; DNA concentration: ? 50 ng/μl; Purity: OD260/280 = 1.8 ? 2.0 without degradation or RNA contamination

TURNAROUND TIME: 15 working days from verification of sample quality (without data analysis); The data analysis turnaround is projectdependent.

RECOMMENDED SEQUENCING DEPTH: ? 30x