de novo ゲノムシーケンス

ヒト全ゲノムシーケンス 次世代シーケンス(ngs)解析

新規ゲノム配列決定では、種についての最初のゲノムマップは系統発生の研究、種の多様性、特定の形質と遺伝子マーカー、および他のゲノミクス研究のマッピングを分析するための貴重な基準配列を提供し、生成されます。

次世代シーケンシング技術の発展に伴い、新規のゲノム配列決定は、より迅速かつ手頃な価格となっており、Novogeneは、この技術の最前線にあります。 Novogeneの創設者、李博士は、大手ゲノミクスの専門家とゲノムアセンブリのためのSOAPdenovoソフトウェアパッケージの主要な開発者です。李博士とNovogeneチームは、新規ゲノム配列上のいくつかの重要な出版物に貢献している、と私たちはあなたのデノボゲノム配列projeに必要な専門知識の高いレベルを提供することができます

サービスの強み

Highly experienced
We have completed major de novogenome sequencing projects, and our data has been published in top-tier journals.
Leader in NGS services
We provide high-quality sequencing, an efficient standard workflow, fast turnaround time, and bioinformatics analyses at a cost-effective price.
Bioinformatics expertise
he SOAPdenovoII software and the NovoHeter software, developed by Novogene scientists, are used for complex genome assembly.

Project Types

Simple Genome
Simple genome refers to a haploid genome with a low repeat content (less than 50%), or a diploid genome with a low rate of heterozygosity (less than 0.5%), such as most mammals, birds, and cultivated crops
Complex Genome
Complex genome refers to a diploid or polyploid genome with a high repeat content (higher than 50%) or a high rate of heterozygosity (higher than 0.5%), such as many species of plants, aquatics, and insects.?Moderately heterozygous genome (diploid)?Highly heterozygous genome (diploid)?Highly repetitive genome (diploid)

サービスの流れ

ヒト全ゲノムシーケンス 次世代シーケンス(ngs)解析の流れ
SEQUENCING STRATEGY
HiSeq platform, paired-end 150 bp
SAMPLE REQUIREMENTS
DNA amount for survey: ≧ 10 μg (quantified by Qubit 2.0)
DNA amount for RAD-seq: ≧ 3 μg (quantified by Qubit 2.0)
          
DNA amount for genome de novo sequencing: ≧ 500 μg (quantified by Qubit 2.0
DNA concentration: ≧ 50 ng/μ
          
Purity: OD260/280 = 1.8 - 2.0 without degradation and RNA contamination
DATA QUALITY GUARANTEE
Simple genome: Contig N50 ≧ 30 Kb, Scaffold N50 ≧ 1 Mb
Mammal or bird genome: Contig N50 ≧ 30 Kb, Scaffold N50 ≧ 3 Mb
Complex genome: Contig N50 ? 20 Kb, Scaffold N50 ? 300 Kb