de novo ゲノムシーケンス

ヒト全ゲノムシーケンス 次世代シーケンス(ngs)解析




Highly experienced
We have completed major de novogenome sequencing projects, and our data has been published in top-tier journals.
Leader in NGS services
We provide high-quality sequencing, an efficient standard workflow, fast turnaround time, and bioinformatics analyses at a cost-effective price.
Bioinformatics expertise
he SOAPdenovoII software and the NovoHeter software, developed by Novogene scientists, are used for complex genome assembly.

Project Types

Simple Genome
Simple genome refers to a haploid genome with a low repeat content (less than 50%), or a diploid genome with a low rate of heterozygosity (less than 0.5%), such as most mammals, birds, and cultivated crops
Complex Genome
Complex genome refers to a diploid or polyploid genome with a high repeat content (higher than 50%) or a high rate of heterozygosity (higher than 0.5%), such as many species of plants, aquatics, and insects.?Moderately heterozygous genome (diploid)?Highly heterozygous genome (diploid)?Highly repetitive genome (diploid)


ヒト全ゲノムシーケンス 次世代シーケンス(ngs)解析の流れ
HiSeq platform, paired-end 150 bp
DNA amount for survey: ≧ 10 μg (quantified by Qubit 2.0)
DNA amount for RAD-seq: ≧ 3 μg (quantified by Qubit 2.0)
DNA amount for genome de novo sequencing: ≧ 500 μg (quantified by Qubit 2.0
DNA concentration: ≧ 50 ng/μ
Purity: OD260/280 = 1.8 - 2.0 without degradation and RNA contamination
Simple genome: Contig N50 ≧ 30 Kb, Scaffold N50 ≧ 1 Mb
Mammal or bird genome: Contig N50 ≧ 30 Kb, Scaffold N50 ≧ 3 Mb
Complex genome: Contig N50 ? 20 Kb, Scaffold N50 ? 300 Kb